Ultrasensitive and rapid identification of ESRI developer- and piperacillin/tazobactam-resistant Escherichia coli by the MALDIpiptaz test.

ABSTRACT:

Background

The excessive use of piperacillin/tazobactam (P/T) has promoted the emergence of P/T-resistant Enterobacterales. We reported that in Escherichia coli, P/T contributes to the development of extended-spectrum resistance to β-lactam/β-lactamase inhibitor (BL/BLI) (ESRI) in isolates that are P/T susceptible but have low-level resistance to BL/BLI. Currently, the detection of P/T resistance relying on conventional methods is time-consuming. To overcome this issue, we developed a cost-effective test based on MALDI-MS technology, called MALDIpiptaz, which aims to detect P/T resistance and ESRI developers in E. coli.

Methods

We used automated Clover MS Data Analysis software to analyse the protein profile spectra obtained by MALDI-MS from a collection

SUBMITTER: Rodriguez Villodres A 

PROVIDER: S-EPMC9423838 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature