Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification

ABSTRACT: Rapid and accurate discrimination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an available approach to implement a rapid diagnosis of the coronavirus disease 2019 (COVID-19). Here we fully exploited the cleavage properties of exonuclease III (Exo III) and hairpin DNA-assisted target cycling technology to generate bulk single-stranded DNA (ssDNA) that was employed to facilitate the constitution of a three-way junction structure on polymetallic particle (Ag-Au NPs) and Ti3C2 (Ti3C2 @Ag-Au) complexes. Ag-Au NPs presented favorable stability without adding extra stabilizers, demonstrating the potential value of Ag-Au NPs as an alternative to Au NPs in the field of bioanalysis. Uppon the three-way junction structure, the dumbbell hybridization chain amplification (DHCA) w

SUBMITTER: Wu B 

PROVIDER: S-EPMC9423879 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature