EV-miRome-wide profiling uncovers miR-320c for detecting metastatic colorectal cancer and monitoring the therapeutic response.

ABSTRACT:

Purpose

Molecular composition of circulating small extracellular vesicles (EVs) does not merely reflect the cells of origin, but also is enriched in specific biomolecules directly associated with the cellular transformation. However, while most of the currently identified EV-miRs are only geared towards one-dimensional disease detection, their application for long-term tracking and treatment response monitoring has been largely elusive.

Methods

We established and optimized a rapid, sensitive and robust liquid biopsy sampling method, and further used small RNA sequencing to comprehensively catalogue EV-miRomes in association with the progression and outcome of metastatic colorectal cancer (mCRC).

Results

By cross-comparison of EV-miRomes (n = 290) from multi-stage and

SUBMITTER: Yang CK 

PROVIDER: S-EPMC9424175 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature