Osteogenic transdifferentiation of primary human fibroblasts to osteoblast-like cells with human platelet lysate.

ABSTRACT: Inherited bone disorders account for about 10% of documented Mendelian disorders and are associated with high financial burden. Their study requires osteoblasts which play a critical role in regulating the development and maintenance of bone tissue. However, bone tissue is not always available from patients. We developed a highly efficient platelet lysate-based approach to directly transdifferentiate skin-derived human fibroblasts to osteoblast-like cells. We extensively characterized our in vitro model by examining the expression of osteoblast-specific markers during the transdifferentiation process both at the mRNA and protein level. The transdifferentiated osteoblast-like cells showed significantly increased expression of a panel of osteogenic markers. Mineral deposition and ALP activit

SUBMITTER: Cayami FK 

PROVIDER: S-EPMC9424276 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature