Preserving avian blood and DNA sampled in the wild: A survey of personal experiences.

ABSTRACT: Collecting and storing biological material from wild animals in a way that does not deteriorate DNA quality for subsequent analyses is instrumental for research in ecology and evolution. Our aims were to gather reports on the effectiveness of methods commonly used by researchers for the field collection and long-term storage of blood samples and DNA extracts from wild birds. Personal experiences were collected with an online survey targeted specifically at researchers sampling wild birds. Many researchers experienced problems with blood sample storage but not with DNA extract storage. Storage issues generated problems with obtaining adequate DNA quality and sufficient DNA quantity for the targeted molecular analyses but were not related to season of blood sampling, access to equipment, tra

SUBMITTER: Di Lecce I 

PROVIDER: S-EPMC9424668 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature