Probing the interaction of superparamagnetic iron oxide nanoparticles with lipase and their interacting consequences at the molecular level.

ABSTRACT:

Background

Although superparamagnetic iron oxide nanoparticles (SPIONs) are used as carriers for candida rugosa lipase (CRL) in biomedical fields, their interactions and the influences on CRL are still unknown. Consequently, SPIONs were synthesized, characterized, and incubated with CRL to explore their molecular interactions and interacting consequences in this study.

Methods

The toxic effects of SPIONs on CRL and their molecular interactions were explored through transmission electron microscope, isothermal titration calorimetry, zeta potential measurements, multi-spectroscopic techniques, and biological enzyme activity tests.

Results

Results revealed the adsorption of SPIONs to CRL and the reduction of CRL aggregation. The unfolding and loosening of CRL stru

SUBMITTER: Yang B 

PROVIDER: S-EPMC9424702 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature