Molecular epidemiology of measles virus infection in Shanghai in 2000-2012: the first appearance of genotype D8.

ABSTRACT:

Purpose

The purpose of this study was to identify measles virus in Shanghai in 2012 and study the genotype trend of measles virus epidemic strains during 2000-2012.

Methods

Nose and throat swab specimens were collected from 34 suspected measles cases in Shanghai. Measles virus was isolated using Vero-SLAM cells (African green monkey kidney cells/lymphoid signal activating factor-transfected African green monkey kidney cells). The 450 bp of C terminus of the N gene and the entire hemagglutinin gene sequence was amplified using RT-PCR. Phylogenetic analysis was performed by comparing the seven measles strains in Shanghai with the reference strains for H1a, H1b and D8 genotypes, as well as the Chinese measles virus vaccine strain.

Results

Seven measles viruses strains we

SUBMITTER: Li S 

PROVIDER: S-EPMC9425214 | biostudies-literature | 2014 Nov-Dec

REPOSITORIES: biostudies-literature