Microarray-based genotyping and detection of drug-resistant HBV mutations from 620 Chinese patients with chronic HBV infection.

ABSTRACT:

Background

Research has shown that hepatitis B virus (HBV) genotypes are closely linked to the clinical manifestations, treatment, and prognosis of the disease.

Objective

To study the association between genotype and drug-resistant HBV mutations in 620 Chinese patients with chronic HBV infection.

Methods

HBV DNA levels were determined using real-time quantitative PCR in plasma samples. Microarrays were performed for the simultaneous detection of HBV genotypes (HBV/B, C, and D) and drug-resistance-related hotspot mutations. A portion of the samples analyzed using microarrays was selected randomly and the data were confirmed using direct DNA sequencing.

Results

Most samples were genotype C (471/620; 76.0%), followed by genotype B (149/620; 24.0%). Among the 620 p

SUBMITTER: Hua W 

PROVIDER: S-EPMC9425391 | biostudies-literature | 2015 May-Jun

REPOSITORIES: biostudies-literature