Data-Independent Acquisition for the Detection of Mononucleoside RNA Modifications by Mass Spectrometry.

ABSTRACT: RNA is dynamically modified in cells by a plethora of chemical moieties to modulate molecular functions and processes. Over 140 modifications have been identified across species and RNA types, with the highest density and diversity of modifications found in tRNA (tRNA). The methods used to identify and quantify these modifications have developed over recent years and continue to advance, primarily in the fields of next-generation sequencing (NGS) and mass spectrometry (MS). Most current NGS methods are limited to antibody-recognized or chemically derivatized modifications and have limitations in identifying multiple modifications simultaneously. Mass spectrometry can overcome both of these issues, accurately identifying a large number of modifications in a single run. Here, we present adva

SUBMITTER: Janssen KA 

PROVIDER: S-EPMC9425428 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature