Glioblastoma CD105⁺ cells define a SOX2^- cancer stem cell-like subpopulation in the pre-invasive niche.

ABSTRACT: Glioblastoma (GBM) is the most common and most aggressive primary brain tumor in adults. Glioma stem like cells (GSC) represent the highest cellular hierarchy in GBM and have a determining role in tumor growth, recurrence and patient prognosis. However, a better definition of GSC subpopulations, especially at the surgical resection margin, is warranted for improved oncological treatment options. The present study interrogated cells expressing CD105 (CD105⁺) specifically within the tumor front and the pre-invasive niche as a potential GSC subpopulation. GBM primary cell lines were generated from patients (n = 18) and CD105⁺ cells were isolated and assessed for stem-like characteristics. In vitro, CD105⁺ cells proliferated and enriched in serum-containing medium but not in serum-free conditions. CD105⁺ cells were characterized by Nestin⁺, Vimentin⁺ and SOX2^-, clearly distinguishing them from SOX2⁺ GCS. GBM CD105⁺ cells differentiated into osteocytes and adipocytes but not chondrocytes. Exome sequencing revealed that GBM CD105⁺ cells matched 83% of somatic mutations in the Cancer cell line encyclopedia, indicating a malignant phenotype and in vivo xenotransplantation assays verified their tumorigenic potential. Cytokine assays showed that immunosuppressive and protumorigenic cytokines such as IL6, IL8, CCL2, CXCL-1 were produced by CD105⁺ cells. Finally, screening for 88 clinical drugs revealed that GBM CD105⁺ cells are resistant to most chemotherapeutics except Doxorubicin, Idarubicin, Fludarabine and ABT-751. Our study provides a rationale for targeting tumoral CD105⁺ cells in order to reshape the tumor microenvironment and block GBM progression.

SUBMITTER: Li J 

PROVIDER: S-EPMC9426031 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature