Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress.

ABSTRACT: Abiotic stresses are major environmental conditions that reduce plant growth, productivity and quality. Protein-protein interaction (PPI) approaches can be used to screen stress-responsive proteins and reveal the mechanisms of protein response to various abiotic stresses. Biotin-based proximity labeling (PL) is a recently developed technique to label proximal proteins of a target protein. TurboID, a biotin ligase produced by directed evolution, has the advantages of non-toxicity, time-saving and high catalytic efficiency compared to other classic protein-labeling enzymes. TurboID-based PL has been successfully applied in animal, microorganism and plant systems, particularly to screen transient or weak protein interactions, and detect spatially or temporally restricted local proteomes in li

SUBMITTER: Zhang K 

PROVIDER: S-EPMC9426856 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature