A scalable and modular automated pipeline for stitching of large electron microscopy datasets.

ABSTRACT: Serial-section electron microscopy (ssEM) is the method of choice for studying macroscopic biological samples at extremely high resolution in three dimensions. In the nervous system, nanometer-scale images are necessary to reconstruct dense neural wiring diagrams in the brain, so -called connectomes. The data that can comprise of up to 10⁸ individual EM images must be assembled into a volume, requiring seamless 2D registration from physical section followed by 3D alignment of the stitched sections. The high throughput of ssEM necessitates 2D stitching to be done at the pace of imaging, which currently produces tens of terabytes per day. To achieve this, we present a modular volume assembly software pipeline ASAP (Assembly Stitching and Alignment Pipeline) that is s

SUBMITTER: Mahalingam G 

PROVIDER: S-EPMC9427110 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature