A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli.

ABSTRACT:

Objectives

Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.

Methods

In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and W

SUBMITTER: Bagheri S 

PROVIDER: S-EPMC9427529 | biostudies-literature | 2014 May-Jun

REPOSITORIES: biostudies-literature