Localized efficacy of environmental RNAi in Tetranychus urticae.

ABSTRACT: Environmental RNAi has been developed as a tool for reverse genetics studies and is an emerging pest control strategy. The ability of environmental RNAi to efficiently down-regulate the expression of endogenous gene targets assumes efficient uptake of dsRNA and its processing. In addition, its efficiency can be augmented by the systemic spread of RNAi signals. Environmental RNAi is now a well-established tool for the manipulation of gene expression in the chelicerate acari, including the two-spotted spider mite, Tetranychus urticae. Here, we focused on eight single and ubiquitously-expressed genes encoding proteins with essential cellular functions. Application of dsRNAs that specifically target these genes led to whole mite body phenotypes-dark or spotless. These phenotypes were associate

SUBMITTER: Bensoussan N 

PROVIDER: S-EPMC9427735 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature