Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran.

ABSTRACT:

Background

Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis.

Methods

All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within.

Results

Humans were found to be infected with L. major with high infection frequency and also L

SUBMITTER: Zahirnia AH 

PROVIDER: S-EPMC9427796 | biostudies-literature | 2018 Jul - Aug

REPOSITORIES: biostudies-literature