Comprehensive chromosome FISH assessment of sperm aneuploidy in normozoospermic males.

ABSTRACT:

Purpose

Sperm chromosomal abnormalities impact male fertility and pregnancy outcomes. However, the proportion of sperm with chromosomal abnormalities in normozoospermic men remains unclear. Herein, we evaluated sperm aneuploidy for 23 chromosomes to elucidate its incidence in normozoospermic men.

Methods

Sperm from ten normozoospermic donors were obtained from a human sperm bank and analyzed using fluorescence in situ hybridization. The frequencies of nullisomy, disomy, and diploidy were analyzed along with trisomy, triploidy, tetraploidy, and other numerical abnormalities per chromosome and per donor levels.

Results

A total of 248,811 sperm cells were analyzed (average: 24,881 ± 381 cells/donor), of which 246, 658 were haploid, 818 nullisomic, 393 disomic, 894 diploi

SUBMITTER: Zhu S 

PROVIDER: S-EPMC9428091 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature