Age-dependent microstructure alterations in 5xFAD mice by high-resolution diffusion tensor imaging.

ABSTRACT:

Purpose

To evaluate the age-dependent microstructure changes in 5xFAD mice using high-resolution diffusion tensor imaging (DTI).

Methods

The 5xFAD mice at 4, 7.5, and 12 months and the wild-type controls at 4 months were scanned at 9.4T using a 3D echo-planar imaging (EPI) pulse sequence with the isotropic spatial resolution of 100 μm. The b-value was 3000 s/mm² for all the diffusion MRI scans. The samples were also acquired with a gradient echo pulse sequence at 50 μm isotropic resolution. The microstructure changes were quantified with DTI metrics, including fractional anisotropy (FA) and mean diffusivity (MD). The conventional histology was performed to validate with MRI findings.

Results

The FA values (p = 0.028) showed significant differe

SUBMITTER: Maharjan S 

PROVIDER: S-EPMC9428354 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature