The introduction of an N-glycosylation site into prochymosin greatly enhances its production and secretion by Pichia pastoris.

ABSTRACT:

Background

N-glycosylation is one of the most important post-translational modifications. Many studies have shown that N-glycosylation has a significant effect on the secretion level of heterologous glycoproteins in yeast cells. However, there have been few studies reporting a clear and unified explanation for the intracellular mechanism that N-glycosylation affect the secretion of heterologous glycoproteins so far. Pichia pastoris is an important microbial cell factory producing heterologous protein. It is of great significance to study the effect of N-glycosylation on the secretion level of heterologous protein. Camel chymosin is a glycoprotein with higher application potential in cheese manufacturing industry. We have expressed camel prochymosin in P. pastoris GS115, but the low

SUBMITTER: Wang N 

PROVIDER: S-EPMC9429577 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature