Whole-transcriptome analysis of periodontal tissue and construction of immune-related competitive endogenous RNA network.

ABSTRACT:

Background

In periodontitis, noncoding RNAs may play a regulatory role in the immune microenvironment through competitive endogenous RNA. We aimed to profile noncoding RNA expression and construct immune-related ceRNA network in periodontitis.

Methods

Five inflamed periodontal tissue and five healthy gingivae were collected for whole-transcriptome sequencing. Differential gene, functional enrichment, and protein-protein interaction network analysis were performed to explore the function of differentially expressed genes. CIBERSORTx was used to analyze level of immune cell infiltration in the periodontal tissue. An immune-related competitive endogenous RNA network was constructed and expression of key regulators in the network was validated.

Results

Compared with healt

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC9429583 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature