Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts.

ABSTRACT:

Background

Symbiotic gut microbes have a rich genomic and metabolic pool and are closely related to hosts' health. Traditional sequencing profiling masks the genomic and phenotypic diversity among strains from the same species. Innovative droplet-based microfluidic cultivation may help to elucidate the inter-strain interactions. A limited number of bacterial phylotypes colonize the honeybee gut, while individual strains possess unique genomic potential and critical capabilities, which provides a particularly good model for strain-level analyses.

Results

Here, we construct a droplet-based microfluidic platform and generated ~ 6 × 10⁸ droplets encapsulated with individual bacterial cells from the honeybee gut and cultivate in different media. Shotgun metagenomic ana

SUBMITTER: Meng Y 

PROVIDER: S-EPMC9429759 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature