Optimisation of ¹H PMLG homonuclear decoupling at 60 kHz MAS to enable ¹⁵N-¹H through-bond heteronuclear correlation solid-state NMR spectroscopy.

ABSTRACT: The Lee-Goldburg condition for homonuclear decoupling in ¹H magic-angle spinning (MAS) solid-state NMR sets the angle θ, corresponding to arctan of the ratio of the rf nutation frequency, ν₁, to the rf offset, to be the magic angle, θ_m, equal to tan^-1(√2) = 54.7°. At 60 kHz MAS, we report enhanced decoupling compared to MAS alone in a ¹H spectrum of ¹⁵N-glycine with at θ = 30° for a ν₁ of ∼100 kHz at a ¹H Larmor frequency, ν₀, of 500 MHz and 1 GHz, corresponding to a high chemical shift scaling factor (λ_CS) of 0.82. At 1 GHz, we also demonstrate enhanced decoupling compared to 60 kHz MAS alone for a lower ν₁ of 51 k

SUBMITTER: Tognetti J 

PROVIDER: S-EPMC9429863 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature