Modulation of dendritic cell metabolism by an MPLA-adjuvanted allergen product for specific immunotherapy.

ABSTRACT:

Background

Recently, bacterial components were shown to enhance immune responses by shifting immune cell metabolism towards glycolysis and lactic acid production, also known as the Warburg Effect. Currently, the effect of allergen products for immunotherapy (AIT) and commercial vaccines on immune cell metabolism is mostly unknown.

Objective

To investigate the effect of AIT products (adjuvanted with either MPLA or Alum) on myeloid dendritic cell (mDC) metabolism and activation.

Methods

Bone marrow-derived mDCs were stimulated with five allergoid-based AIT products (one adjuvanted with MPLA, four adjuvanted with Alum) and two MPLA-adjuvanted vaccines and analyzed for their metabolic activation, expression of cell surface markers, and cytokine secretion by ELISA. mDCs we

SUBMITTER: Zimmermann J 

PROVIDER: S-EPMC9430023 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature