Development and Application of Two Inducible Expression Systems for Streptococcus suis.

ABSTRACT: Streptococcus suis is an important zoonotic bacterial pathogen posing a threat to the pig industry as well as public health, for which the mechanisms of growth and cell division remain largely unknown. Developing convenient genetic tools that can achieve strictly controlled gene expression is of great value for investigating these fundamental physiological processes of S. suis. In this study, we first identified three strong constitutive promoters, P_g, P_t, and P_e, in S. suis. Promoter P_g was used to drive the expression of repressor genes tetR and lacI, and the operator sequences were added within promoters P_t and P_e. By optimizing the insertion sites of the operator sequence, we successfully constructed an anh

SUBMITTER: Zhang L 

PROVIDER: S-EPMC9430170 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature