How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions.

ABSTRACT: Dimethyl sulfoxide (DMSO) increases cell and tissue viability at low temperatures and is commonly used as a cryoprotectant for cryogenic storage of biological materials. DMSO disorders the water hydrogen-bond networks and inhibits ice-crystal growth, though the specific DMSO interactions with water are difficult to characterize. In this study, we use a combination of Fourier Transform infrared spectroscopy (FTIR), molecular dynamics simulations, and vibrational frequency maps to characterize the temperature-dependent hydrogen bonding interactions of DMSO with water from 30 °C to -80 °C. Specifically, broad peaks in O-D stretch vibrational spectra of DMSO and deuterated water (HDO) cosolvent systems show that the hydrogen bond networks become increasingly disrupted compared to pure water. S

SUBMITTER: Lee E 

PROVIDER: S-EPMC9430440 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature