Isoenzyme N-Acyl-l-Amino Acid Amidohydrolase NA Increases Ochratoxin A Degradation Efficacy of Stenotrophomonas sp. CW117 by Enhancing Amidohydrolase ADH3 Stability.

ABSTRACT: Ochratoxin A (OTA) is a potent mycotoxin mainly produced by toxicogenic strains of Aspergillus spp. and seriously contaminates foods and feedstuffs. OTA detoxification strategies are significant to food safety. A superefficient enzyme ADH3 to OTA hydrolysis was isolated from the difunctional strain Stenotrophomonas sp. CW117 in our previous study. Here, we identified a gene N-acyl-l-amino acid amidohydrolase NA, which is an isoenzyme of ADH3. However, it is not as efficient a hydrolase as ADH3. The kinetic constant showed that the catalytic efficiency of ADH3 (K_cat/K_m = 30,3938 s^-1 · mM^-1) against OTA was 29,113 times higher than that of NA (K_cat/K_m = 10.4 s^-1 · mM<

SUBMITTER: Chen N 

PROVIDER: S-EPMC9430628 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature