LI-Detector: a Method for Curating Ordered Gene-Replacement Libraries.

ABSTRACT: In recent years the availability of genome sequence information has grown logarithmically resulting in the identification of a plethora of uncharacterized genes. To address this gap in functional annotation, many high-throughput screens have been devised to uncover novel gene functions. Gene-replacement libraries are one such tool that can be screened in a high-throughput way to link genotype and phenotype and are key community resources. However, for a phenotype to be attributed to a specific gene, there needs to be confidence in the genotype. Construction of large libraries can be laborious and occasionally errors will arise. Here, we present a rapid and accurate method for the validation of any ordered library where a gene has been replaced or disrupted by a uniform linear insertion (LI

SUBMITTER: Goodall ECA 

PROVIDER: S-EPMC9431181 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature