A Cytoplasmic NAD(P)H-Dependent Polysulfide Reductase with Thiosulfate Reductase Activity from the Hyperthermophilic Bacterium Thermotoga maritima.

ABSTRACT: Thermotoga maritima is an anaerobic hyperthermophilic bacterium that efficiently produces H₂ by fermenting carbohydrates. High concentration of H₂ inhibits the growth of T. maritima, and S⁰ could eliminate the inhibition and stimulate the growth through its reduction. The mechanism of T. maritima sulfur reduction, however, has not been fully understood. Herein, based on its similarity with archaeal NAD(P)H-dependent sulfur reductases (NSR), the ORF THEMA_RS02810 was identified and expressed in Escherichia coli, and the recombinant protein was characterized. The purified flavoprotein possessed NAD(P)H-dependent S⁰ reductase activity (1.3 U/mg for NADH and 0.8 U/mg for NADPH), polysulfide reductase activity (0.32 U/mg for NADH and 0.35 U/mg for NAD

SUBMITTER: Liang J 

PROVIDER: S-EPMC9431562 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature