Short Signature rpoB Gene Sequence to Differentiate Species in Mycobacterium abscessus Group.

ABSTRACT: Mycobacterium abscessus group (MAG) are rapidly growing acid-fast bacteria that consist of three closely related species: M. abscessus (Ma), M. bolletii (Mb), and M. massiliense (Mm). Differentiation of these species can be difficult but is increasingly requested owing to recent infectious outbreaks and their differential drug resistance. We developed a novel and rapid pyrosequencing method using short signature sequences (35 to 45 bp) at a hypervariable site in the rpoB gene to differentiate the three MAG species, along with M. chelonae (Mc), and M. immunogenum (Mi). This method was evaluated using 111 M. chelonae-abscessus complex (MCAC) isolates, including six reference strains. All isolates were successfully differen

SUBMITTER: Bao JR 

PROVIDER: S-EPMC9431568 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature