Functional Genomics Identified Novel Genes Involved in Growth at Low Temperatures in Listeria monocytogenes.

ABSTRACT: Listeria monocytogenes (Lm) is a foodborne pathogen that can cause severe human illness. Standard control measures for restricting bacterial growth, such as refrigeration, are often inadequate as Lm grows well at low temperatures. To identify genes involved in growth at low temperatures, a powerful functional genomics method Tn-seq was performed in this study. This genome-wide screening comprehensively identified the known and novel genetic determinants involved in low-temperature growth. A novel gene lmo1366, encoding rRNA methyltransferase, was identified to play an essential role in Lm growth at 16°C. In contrast, the inactivation of lmo2301, a gene encoding the terminase of phage A118, significantly enhanced the growth of Lm at 16°C. The deletion of lmo1366 or l

SUBMITTER: Wu Y 

PROVIDER: S-EPMC9431668 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature