Association between interleukin-10 polymorphisms and CD4⁺CD25⁺FOXP3⁺ T cells in asthmatic children.

ABSTRACT:

Objective

The aim of this study was to evaluate the association between possible functional interleukin-10 (IL-10) polymorphisms, IL-10 expression and regulatory T cells (Tregs) frequency, and/or asthma severity in a sample of children and adolescents.

Methods

This is a nested case-control genetic association study. The study sample consisted of children and adolescents aged 8-14 from public schools. Four polymorphisms of the IL-10 gene (rs1518111, rs3024490, rs3024496, rs3024491) were genotyped in asthmatic subjects and controls using real-time PCR. Tregs cells and IL-10 were analyzed in peripheral blood mononuclear cells by flow cytometry. The severity of asthma was defined according to the Global Initiative for Asthma (GINA) guideline.

Results

One hundred twenty-th

SUBMITTER: Mocellin M 

PROVIDER: S-EPMC9432050 | biostudies-literature | 2021 Sep-Oct

REPOSITORIES: biostudies-literature