Role of C3a as a Novel Regulator of 25(OH)D₃ to 1α,25-Dihydroxyvitamin D₃ Metabolism in Upper Airway Epithelial Cells.

ABSTRACT: In patients with chronic rhinosinusitis with nasal polyps, primary human sinonasal epithelial cell (HSNEC) 1α-hydroxylase levels are reduced, as is their ability to metabolize 25-hydroxycholecalciferol [25(OH)D₃] to its active metabolite, 1α,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃]. In this study, we sought to identify the factor responsible for the regulation of HSNEC metabolism of 25(OH)D₃, focusing on C3 and C3a. Multiple inhaled irritants trigger the release of complement components, C3 and C3a, leading to suppression of 1α-hydroxylase levels in HSNECs. Recombinant C3a was able to decrease 1α-hydroxylase and impair 25(OH)D₃ to 1,25(OH)₂D₃ metabolism, while addition of a C3a receptor antagonist restored

SUBMITTER: Mulligan JK 

PROVIDER: S-EPMC9432430 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature