An optimized method of extracting and quantifying active Neutrophil serine proteases from human whole blood cells.

ABSTRACT:

Purpose

Neutrophil serine proteases (NSPs) are implicated in numerous inflammatory diseases. Thus, a robust methodology to monitor and quantify NSPs is important to study disease progression and evaluate the effect of pharmacological interventions. A comparison of the various methods used to extract NSPs from neutrophil granulocytes has not been published, providing the impetus to conduct this method optimization and comparison study.

Methods

Two NSP recovery methodologies were evaluated on samples from five human donors: zymosan stimulation and cell pellet extraction. For the zymosan stimulation method, 1 mL donor blood was added to zymosan and samples were incubated at 37°C for 30 min while shaking. Samples were then centrifuged, and the plasma was collected for quantitati

SUBMITTER: Basso J 

PROVIDER: S-EPMC9432755 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature