Dataset Information

Optical coherence tomography holds promise to transform the diagnostic anatomic pathology gross evaluation process.

ABSTRACT:

Significance

Real-time histology can close a variety of gaps in tissue diagnostics. Currently, gross pathology analysis of excised tissue is dependent upon visual inspection and palpation to identify regions of interest for histopathological processing. Such analysis is limited by the variable correlation between macroscopic and microscopic findings. The current standard of care is costly, burdensome, and inefficient.

Aim

We are the first to address this gap by introducing optical coherence tomography (OCT) to be integrated in real-time during the pathology grossing process.

Approach

This is achieved by our high-resolution, ultrahigh-speed, large field-of-view OCT device designed for this clinical application.

Results

We demonstrate the feasibility of imaging tissue sections from multiple human organs (breast, prostate, lung, and pancreas) in a clinical gross pathology setting without interrupting standard workflows.

Conclusions

OCT-based real-time histology evaluation holds promise for addressing a gap that has been present for >100 years.

SUBMITTER: Mojahed D

PROVIDER: S-EPMC9434002 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Publications

Optical coherence tomography holds promise to transform the diagnostic anatomic pathology gross evaluation process.

Mojahed Diana D Applegate Matthew M Guo Hua H Taback Bret B Ha Richard R Hibshoosh Hanina H Hendon Christine C

Journal of biomedical optics 20220901 9

<h4>Significance</h4>Real-time histology can close a variety of gaps in tissue diagnostics. Currently, gross pathology analysis of excised tissue is dependent upon visual inspection and palpation to identify regions of interest for histopathological processing. Such analysis is limited by the variable correlation between macroscopic and microscopic findings. The current standard of care is costly, burdensome, and inefficient.<h4>Aim</h4>We are the first to address this gap by introducing optical ...[more]

PMID: 36050827

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Project description:BackgroundCerebral small vessel disease (CSVD) is a disorder of brain vasculature that causes various structural changes in the brain parenchyma, and is associated with various clinical symptoms such as cognitive impairment and gait disorders. Structural changes of brain arterioles cannot be visualized with routine imaging techniques in vivo. However, optical coherence tomography (OCT) is thought to be a "window to the brain". Thus, retinal vessel parameters may correlate with CSVD characteristic brain lesions and cerebrospinal fluid biomarkers (CSF) of the neuropathological processes in CSVD like endothelial damage, microglial activation and neuroaxonal damage.MethodsWe applied OCT-based assessment of retinal vessels, magnetic resonance imaging (MRI), and CSF biomarker analysis in a monocentric prospective cohort of 24 patients with sporadic CSVD related stroke and cognitive impairment. MRI lesions were defined according to the STandards for ReportIng Vascular changes on nEuroimaging (STRIVE). Biomarkers were assessed using commercially available ELISA kits. Owing to the unavailability of an age-matched control-group lacking MRI-characteristics of CSVD, we compared the retinal vessel parameters in CSVD patients (73.8 ± 8.5 years) with a younger group of healthy controls (51.0 ± 16.0 years) by using an age- and sex-adjusted multiple linear regression analysis model.ResultsAmong the parameters measured with OCT, the Wall to Lumen Ratio (WLR) but not Mean Wall Thickness (MWT) of the superior branch of the retinal artery correlated significantly with the volume of white matter hyperintensities on MRI (rs = - 0.5) and with CSF-levels of Chitinase 3 like 1 protein (rs = - 0.6), zona occludens 1 protein (rs = - 0.5) and GFAP (rs = - 0.4). MWT and WLR were higher in CSVD than in controls (28.9 μm vs. 23.9 μm, p = 0.001 and 0.32 vs. 0.25, p = 0.001).ConclusionsIn this exploratory study, WLR correlated with the volume of white matter hyperintensities, and markers of vascular integrity, microglial activation, and neuroaxonal damage in CSVD. Further prospective studies should clarify whether retinal vessel parameters and CSF biomarkers may serve to monitor the natural course and treatment effects in clinical studies on CSVD.

Dataset Information

Optical coherence tomography holds promise to transform the diagnostic anatomic pathology gross evaluation process.

Significance

Aim

Approach

Results

Conclusions

Publications

Optical coherence tomography holds promise to transform the diagnostic anatomic pathology gross evaluation process.

Similar Datasets

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