Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.

ABSTRACT: In Schizosaccharomyces pombe, systematic analyses of single transcription factor deletion or overexpression strains have made substantial advances in determining the biological roles and target genes of transcription factors, yet these characteristics are still relatively unknown for over a quarter of them. Moreover, the comprehensive list of proteins that regulate transcription factors remains incomplete. To further characterize Schizosaccharomyces pombe transcription factors, we performed synthetic sick/lethality and synthetic dosage lethality screens by synthetic genetic array. Examination of 2,672 transcription factor double deletion strains revealed a sick/lethality interaction frequency of 1.72%. Phenotypic analysis of these sick/lethality strains revealed potential cell cycle roles

SUBMITTER: Chatfield-Reed K 

PROVIDER: S-EPMC9434175 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature