A comparative transcriptomics and eQTL approach identifies SlWD40 as a tomato fruit ripening regulator.

ABSTRACT: Although multiple vital genes with strong effects on the tomato (Solanum lycopersicum) ripening process have been identified via the positional cloning of ripening mutants and cloning of ripening-related transcription factors (TFs), recent studies suggest that it is unlikely that we have fully characterized the gene regulatory networks underpinning this process. Here, combining comparative transcriptomics and expression QTLs, we identified 16 candidate genes involved in tomato fruit ripening and validated them through virus-induced gene silencing analysis. To further confirm the accuracy of the approach, one potential ripening regulator, SlWD40 (WD-40 repeats), was chosen for in-depth analysis. Co-expression network analysis indicated that master regulators such as RIN (ripening inhibitor)

SUBMITTER: Zhu F 

PROVIDER: S-EPMC9434188 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature