Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes.
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ABSTRACT:
SUBMITTER: Gorecki K
PROVIDER: S-EPMC9434548 | biostudies-literature | 2022 Aug
REPOSITORIES: biostudies-literature
Publications
Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes.
Górecki Kamil K Hansen Jesper S JS Li Ping P Nayeri Niloofar N Lindkvist-Petersson Karin K Gourdon Pontus P
Analytical chemistry 20220815 34
Measurement of protein-facilitated copper flux across biological membranes is a considerable challenge. Here, we demonstrate a straightforward microfluidic-derived approach for visualization and measurement of membranous Cu flux. Giant unilamellar vesicles, reconstituted with the membrane protein of interest, are prepared, surface-immobilized, and assessed using a novel quencher-sensor reporter system for detection of copper. With the aid of a syringe pump, the external buffer is exchanged, enab ...[more]
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