Metabolic state switches between morning and evening in association with circadian clock in people without diabetes.

ABSTRACT:

Aims/introduction

Understanding morning-evening variation in metabolic state is critical for managing metabolic disorders. We aimed to characterize this variation from the viewpoints of insulin secretion and insulin sensitivity, including their relevance to the circadian rhythm.

Materials and methods

A total of 14 and 10 people without diabetes were enrolled, and underwent a 75-g oral glucose tolerance test (OGTT) and hyperinsulinemic-euglycemic clamp study, respectively. Participants completed the OGTT or hyperinsulinemic-euglycemic clamp at 08.00 hours and 20.00 hours in random order. Before each study, hair follicles were collected. In mice, phosphorylation levels of protein kinase B were examined in the liver and muscle by western blotting.

Results

Glucose toleran

SUBMITTER: Fujimoto R 

PROVIDER: S-EPMC9434593 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature