Importance of RNA length for in vitro encapsidation by the nucleoprotein of human respiratory syncytial virus.

ABSTRACT: Respiratory syncytial virus has a negative-sense single-stranded RNA genome constitutively encapsidated by the viral nucleoprotein N, forming a helical nucleocapsid which is the template for viral transcription and replication by the viral polymerase L. Recruitment of L onto the nucleocapsid depends on the viral phosphoprotein P, which is an essential L cofactor. A prerequisite for genome and antigenome encapsidation is the presence of the monomeric, RNA-free, neosynthesized N protein, named N⁰. Stabilization of N⁰ depends on the binding of the N-terminal residues of P to its surface, which prevents N oligomerization. However, the mechanism involved in the transition from N⁰-P to nucleocapsid assembly, and thus in the specificity of viral genome encapsidati

SUBMITTER: Gonnin L 

PROVIDER: S-EPMC9436823 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature