The proliferative and multipotent epidermal progenitor cells for human skin reconstruction in vitro and in vivo.

ABSTRACT:

Objectives

The skin exhibits tremendous regenerative potential, as different types of progenitor and stem cells regulate skin homeostasis and damage. However, in vitro primary keratinocytes present with several drawbacks, such as high donor variability, short lifespan, and limited donor tissue availability. Therefore, more stable primary keratinocytes are needed to generate multiple uniform in vitro and in vivo skin models.

Results

We identified epidermal progenitor cells from primary keratinocytes using Integrin beta 1 (ITGB1) an epidermal stem cell marker markedly decreased after senescence in vitro. Epidermal progenitor cells exhibited unlimited proliferation and the potential for multipotent differentiation capacity. Moreover, they could completely differentiate to form

SUBMITTER: Lim JH 

PROVIDER: S-EPMC9436902 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature