Opposite roles of Kindlin orthologs in cell survival and proliferation.

ABSTRACT:

Objective

It is unclear why adhesion-dependent cells such as epithelium undergo anoikis without anchorage, while adhesion-independent blood cells thrive in suspension. The adhesive machinery of these cells is similar, with the exception of Kindlin orthologs, Kindlin 2 (K2) and Kindlin 3 (K3). Here we address how Kindlins control cell survival and proliferation in anchorage-dependent and independent cells.

Material and methods

To demonstrate the opposite roles of Kindlin's in cell survival we utilized in vivo and in vitro models and K3 and K2 knockdown and knockin cells. We used human lymphocytes from the K3 deficient patients in tumour model, K3 knockout and knockin macrophages and K2 knockout and knockin MEF cells for experiments in under conditions of adhesion and in suspe

SUBMITTER: Zhevlakova I 

PROVIDER: S-EPMC9436913 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature