Heterogeneity and transcriptome changes of human CD8⁺ T cells across nine decades of life.

ABSTRACT: The decline of CD8⁺ T cell functions contributes to deteriorating health with aging, but the mechanisms that underlie this phenomenon are not well understood. We use single-cell RNA sequencing with both cross-sectional and longitudinal samples to assess how human CD8⁺ T cell heterogeneity and transcriptomes change over nine decades of life. Eleven subpopulations of CD8⁺ T cells and their dynamic changes with age are identified. Age-related changes in gene expression result from changes in the percentage of cells expressing a given transcript, quantitative changes in the transcript level, or a combination of these two. We develop a machine learning model capable of predicting the age of individual cells based on their transcriptomic features, which are close

SUBMITTER: Lu J 

PROVIDER: S-EPMC9436929 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature