Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli.

ABSTRACT: Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of ol

SUBMITTER: Zhu KW 

PROVIDER: S-EPMC9437035 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature