In vivo multiphoton multiparametric 3D quantification of human skin aging on forearm and face.

ABSTRACT: Quantifying skin aging changes and characterizing its 3D structure and function in a non-invasive way is still a challenging area of research, constantly evolving with the development of imaging methods and image analysis tools. In vivo multiphoton imaging offers means to assess skin constituents in 3D, however prior skin aging studies mostly focused on 2D analyses of dermal fibers through their signals' intensities or densities. In this work, we designed and implemented multiphoton multiparametric 3D quantification tools for in vivo human skin pigmentation and aging characterization. We first demonstrated that despite the limited field of view of the technic, investigation of 2 regions of interest (ROIs) per zone per volunteer is a good compromise in assessing 3D skin constituents in both

SUBMITTER: Pena AM 

PROVIDER: S-EPMC9437074 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature