CX3CL1-induced CD16⁺ monocytes extravasation in myeloperoxidase-ANCA-associated vasculitis correlates with renal damage.

ABSTRACT:

Background

Monocytes are involved in the pathogenesis of ANCA-associated vasculitis (AAV). Monocyte/macrophages are the dominant infiltrating cells in the glomeruli of patients with myeloperoxidase-AAV (MPO-AAV). However, how human monocyte subsets extravasate to the kidney in MPO-AAV with renal damage is unclear.

Methods

30 MPO-AAV patients with renal damage and 22 healthy controls were enrolled in this study. Monocyte subsets and monocyte-related chemokines in the blood and kidneys of MPO-AAV patients were detected. The chemoattractant activity of the CX3CL1-CX3CR1 axis on CD16⁺ monocytes was observed. The effect of MPO-ANCA on the migration of CD16⁺ monocytes to human glomerular endothelial cells (HGECs) was detected by flow cytometry and transwell m

SUBMITTER: Tang J 

PROVIDER: S-EPMC9437287 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature