Monocytes acquire prostate cancer specific chromatin conformations upon indirect co-culture with prostate cancer cells.

ABSTRACT:

Background

Three-dimensional chromosome loop conformations are powerful regulators of gene expression. These chromosome conformations can be detected both in tumour and in circulating cells and have significant disease biomarker potential. We have recently detected specific chromosome conformations in circulating cells of patients with prostate cancer (PCa) which were similar to ones found in their primary tumours, however, the possibility of horizontal transfer of chromosome conformations was not studied previously.

Methods

Human monocytes (U937) were co-cultured in Boyden chambers through 0.4 uM membrane with or without PC-3 human PCa cells or their conditioned media and a custom DNA microarray for 900,000 chromosomal loops covering all coding loci and non-coding RNA genes

SUBMITTER: Alshaker H 

PROVIDER: S-EPMC9437316 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature