Centaur antibodies: Engineered chimeric equine-human recombinant antibodies.

ABSTRACT: Hyper-immune antisera from large mammals, in particular horses, are routinely used for life-saving anti-intoxication intervention. While highly efficient, the use of these immunotherapeutics is complicated by possible recipient reactogenicity and limited availability. Accordingly, there is an urgent need for alternative improved next-generation immunotherapies to respond to this issue of high public health priority. Here, we document the development of previously unavailable tools for equine antibody engineering. A novel primer set, EquPD v2020, based on equine V-gene data, was designed for efficient and accurate amplification of rearranged horse antibody V-segments. The primer set served for generation of immune phage display libraries, representing highly diverse V-gene repertoires of ho

SUBMITTER: Rosenfeld R 

PROVIDER: S-EPMC9437483 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature