Comprehensive analysis of m⁶A circRNAs identified in colorectal cancer by MeRIP sequencing.

ABSTRACT:

Purpose

To characterize the entire profile of m⁶A modifications and differential expression patterns for circRNAs in colorectal cancer (CRC).

Methods

First, High-throughput MeRIP-sequencing and RNA-sequencing was used to determine the difference in m⁶A methylome and expression of circRNA between CRC tissues and tumor-adjacent normal control (NC) tissues. Then, GO and KEGG analysis detected pathways involved in differentially methylated and differentially expressed circRNAs (DEGs). The correlations between m⁶A status and expression level were calculated using a Pearson correlation analysis. Next, the networks of circRNA-miRNA-mRNA were visualized using the Target Scan and miRanda software. Finally, We describe the relationship of distance betw

SUBMITTER: He F 

PROVIDER: S-EPMC9437624 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature