Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.

ABSTRACT: Insulin secreted from islets of Langerhans is the main hormone to reduce blood glucose. Examination of insulin secretion patterns at the single islet level reveals functional differences in the timings and patterns of release. This heterogeneous response highlights the importance of developing systems to measure dynamic release from small numbers of islets in parallel. Toward this, we describe fluorescence anisotropy imaging immunoassays as a relatively simple method for increased throughput of islet secretion measurements. In this system, vacuum pressure from a syringe pump pulled perfusate from 12 islet chambers and reagents into 12 parallel mixing channels for a competitive immunoassay. Light from a Xe arc lamp was filtered and polarized prior to focusing on the microfluidic device at t

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9437875 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature