Core-shell encapsulation formulations to stabilize desiccated Bradyrhizobium against high environmental temperature and humidity.

ABSTRACT: Engineered materials to improve the shelf-life of desiccated microbial strains are needed for cost-effective bioaugmentation strategies. High temperatures and humidity of legume-growing regions challenge long-term cell stabilization at the desiccated state. A thermostable xeroprotectant core and hydrophobic water vapour barrier shell encapsulation technique was developed to protect desiccated cells from the environment. A trehalose core matrix increased the stability of desiccated Bradyrhizobium by three orders of magnitude over 20 days at 32°C and 50% relative humidity (RH) compared to buffer alone; however, the improvement was not deemed sufficient for a shelf-stable bioproduct. We tested common additives (skim milk, albumin, gelatin and dextran) to increase the glass transition temperat

SUBMITTER: Aukema KG 

PROVIDER: S-EPMC9437883 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature