Optically Responsive Protein Coating of DNA Origami for Triggered Antigen Targeting.

ABSTRACT: DNA nanostructures have emerged as modular building blocks in several research fields including biomedicine and nanofabrication. Their proneness to degradation in various environments has led to the development of a variety of nature-inspired protection strategies. Coating of DNA origami nanostructures with proteins can circumvent degradation and alter their properties. Here, we have used a single-chain variable antibody fragment and serum albumin to construct positively charged and stimuli-responsive protein-dendron conjugates, which were complexed with DNA origami through electrostatic interactions. Using a stepwise assembly approach, the coated nanostructures were studied for their interaction with the corresponding antigen in fluorescence-based immunoassays. The results suggest that th

SUBMITTER: Seitz I 

PROVIDER: S-EPMC9437894 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature